BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//王进的个人网站 - ECPv5.10.0//NONSGML v1.0//EN
CALSCALE:GREGORIAN
METHOD:PUBLISH
X-WR-CALNAME:王进的个人网站
X-ORIGINAL-URL:https://www.jingege.wang
X-WR-CALDESC:Events for 王进的个人网站
BEGIN:VTIMEZONE
TZID:Asia/Shanghai
BEGIN:STANDARD
TZOFFSETFROM:+0800
TZOFFSETTO:+0800
TZNAME:CST
DTSTART:20200101T000000
END:STANDARD
END:VTIMEZONE
BEGIN:VEVENT
DTSTART;TZID=Asia/Shanghai:20220712T080000
DTEND;TZID=Asia/Shanghai:20220712T103000
DTSTAMP:20260406T001321
CREATED:20220706T114551Z
LAST-MODIFIED:20220706T114551Z
UID:7824-1657612800-1657621800@www.jingege.wang
SUMMARY:第72批博士后科学基金线上辅导会议
DESCRIPTION:7月12日上午8：30-10：30通过腾讯会议（会议号377568177）举行召开第72批博士后科学基金线上辅导会议，建议你参会（能够在8月31日前入职的都可以申请）；如果可以参会，请务必提前10分钟进入视频会议！谢谢！如果申请博后项目，请务必在在8月15日前在系统内提交申报材料
URL:https://www.jingege.wang/event/%e7%ac%ac72%e6%89%b9%e5%8d%9a%e5%a3%ab%e5%90%8e%e7%a7%91%e5%ad%a6%e5%9f%ba%e9%87%91%e7%ba%bf%e4%b8%8a%e8%be%85%e5%af%bc%e4%bc%9a%e8%ae%ae/
CATEGORIES:博新计划
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Shanghai:20220201T080000
DTEND;TZID=Asia/Shanghai:20220201T170000
DTSTAMP:20260406T001321
CREATED:20211130T145132Z
LAST-MODIFIED:20211130T145319Z
UID:6657-1643702400-1643734800@www.jingege.wang
SUMMARY:停止给药8周
DESCRIPTION:各组大鼠分别于染毒结束5天、4周和8周后\,用10 %水合氯醛麻醉后经腹主动脉放血处死。\n6只
URL:https://www.jingege.wang/event/%e5%81%9c%e6%ad%a2%e7%bb%99%e8%8d%af8%e5%91%a8/
CATEGORIES:碳化钨
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Shanghai:20220107T130000
DTEND;TZID=Asia/Shanghai:20220107T170000
DTSTAMP:20260406T001321
CREATED:20211130T145025Z
LAST-MODIFIED:20220105T021959Z
UID:6655-1641560400-1641574800@www.jingege.wang
SUMMARY:停止给药4周
DESCRIPTION:各组大鼠分别于染毒结束5天、4周和8周后\,用10 %水合氯醛麻醉后经腹主动脉放血处死。\n6只
URL:https://www.jingege.wang/event/%e5%81%9c%e6%ad%a2%e7%bb%99%e8%8d%af4%e5%91%a8/
CATEGORIES:碳化钨
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Shanghai:20211212T080000
DTEND;TZID=Asia/Shanghai:20211212T170000
DTSTAMP:20260406T001321
CREATED:20211130T144834Z
LAST-MODIFIED:20211209T101550Z
UID:6653-1639296000-1639328400@www.jingege.wang
SUMMARY:停止给药5天
DESCRIPTION:工作安排： \n1、  水合氯醛麻醉（8%，1.5ml），大鼠称重记录； \n2、  静脉采血：2ml 柠檬酸钠抗凝管用于FACS 血常规分析裂红后加冻存液冻存 \n5ml 于血清管分血清用于血生化（ELISA）-80℃保存 \n3、  腹主动脉放血； \n4、  BALF样本制备： \n1)    打开胸腔，暴露肺；用细线结扎右主支气管； \n2)    剪开颈部皮肤，钝性剥离肌肉，找到气管；（需准备剪刀，镊子，止血钳） \n3)    用细线结扎近口端气管； \n4)    用注射器针头刺穿气管，小心插入预制导管，进气管约1cm，用细线扎住导管以稳定； \n5)    注射器吸20ml PBS缓冲液，将 PBS慢慢注入大鼠肺脏，见左肺膨起，缓慢回抽，反复5次，回收BALF至EP管，冰浴； \n6)    在4 ℃下以1500 r/min离心15 min，取上清于-20 ℃冰箱保存备用，如须保存3个月以上，则需于-70℃冰箱储存； \n7)    上清液用做可溶性成分（白蛋白、总蛋白、IL-6和TNF-α）的检测； \n8)    细胞沉淀加入2 ml细胞冻存液，保存于-80℃冰箱，后复苏用于流式分类计数。 \n  \n  \n5、  器官组织样本 \n1)    大鼠处死后，用无菌眼科剪分离肺脏、心脏、肝脏、脾脏、肾脏、胸腺、睾丸； \n吸取器官表面的血污后，称取器官重量，记录数据； \n2)    剪取约0.2- 0.5 g准确称重后置于EP管中，保存于-20 ℃冰箱中，用于测量器官内的钨浓度。 \n\n大鼠编号： \n对照组：1101-1106 \n实验组：2101-2106
URL:https://www.jingege.wang/event/%e5%81%9c%e6%ad%a2%e7%bb%99%e8%8d%af5%e5%a4%a9/
CATEGORIES:碳化钨
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Shanghai:20211207T080000
DTEND;TZID=Asia/Shanghai:20211207T170000
DTSTAMP:20260406T001321
CREATED:20211130T144356Z
LAST-MODIFIED:20211130T144356Z
UID:6651-1638864000-1638896400@www.jingege.wang
SUMMARY:第八次给药
DESCRIPTION:
URL:https://www.jingege.wang/event/%e7%ac%ac%e5%85%ab%e6%ac%a1%e7%bb%99%e8%8d%af/
CATEGORIES:碳化钨
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Shanghai:20211203T080000
DTEND;TZID=Asia/Shanghai:20211203T170000
DTSTAMP:20260406T001321
CREATED:20211130T144326Z
LAST-MODIFIED:20211130T144326Z
UID:6649-1638518400-1638550800@www.jingege.wang
SUMMARY:第七次给药
DESCRIPTION:
URL:https://www.jingege.wang/event/%e7%ac%ac%e4%b8%83%e6%ac%a1%e7%bb%99%e8%8d%af/
CATEGORIES:碳化钨
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Shanghai:20211130T080000
DTEND;TZID=Asia/Shanghai:20211130T170000
DTSTAMP:20260406T001321
CREATED:20211130T144303Z
LAST-MODIFIED:20211130T144303Z
UID:6647-1638259200-1638291600@www.jingege.wang
SUMMARY:第六次给药
DESCRIPTION:
URL:https://www.jingege.wang/event/%e7%ac%ac%e5%85%ad%e6%ac%a1%e7%bb%99%e8%8d%af/
CATEGORIES:碳化钨
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Shanghai:20211126T080000
DTEND;TZID=Asia/Shanghai:20211126T170000
DTSTAMP:20260406T001321
CREATED:20211130T144241Z
LAST-MODIFIED:20211130T144241Z
UID:6645-1637913600-1637946000@www.jingege.wang
SUMMARY:第五次给药
DESCRIPTION:
URL:https://www.jingege.wang/event/%e7%ac%ac%e4%ba%94%e6%ac%a1%e7%bb%99%e8%8d%af/
CATEGORIES:碳化钨
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Shanghai:20211123T080000
DTEND;TZID=Asia/Shanghai:20211123T170000
DTSTAMP:20260406T001321
CREATED:20211130T144208Z
LAST-MODIFIED:20211130T144208Z
UID:6643-1637654400-1637686800@www.jingege.wang
SUMMARY:第四次给药
DESCRIPTION:
URL:https://www.jingege.wang/event/%e7%ac%ac%e5%9b%9b%e6%ac%a1%e7%bb%99%e8%8d%af/
CATEGORIES:碳化钨
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Shanghai:20211119T080000
DTEND;TZID=Asia/Shanghai:20211119T170000
DTSTAMP:20260406T001321
CREATED:20211130T144139Z
LAST-MODIFIED:20211130T144139Z
UID:6641-1637308800-1637341200@www.jingege.wang
SUMMARY:第一次给药
DESCRIPTION:
URL:https://www.jingege.wang/event/%e7%ac%ac%e4%b8%80%e6%ac%a1%e7%bb%99%e8%8d%af-2/
CATEGORIES:碳化钨
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Shanghai:20211116T080000
DTEND;TZID=Asia/Shanghai:20211116T170000
DTSTAMP:20260406T001321
CREATED:20211130T144117Z
LAST-MODIFIED:20211130T144117Z
UID:6639-1637049600-1637082000@www.jingege.wang
SUMMARY:第二次给药
DESCRIPTION:
URL:https://www.jingege.wang/event/%e7%ac%ac%e4%ba%8c%e6%ac%a1%e7%bb%99%e8%8d%af/
CATEGORIES:碳化钨
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Shanghai:20211112T080000
DTEND;TZID=Asia/Shanghai:20211112T170000
DTSTAMP:20260406T001321
CREATED:20211130T144048Z
LAST-MODIFIED:20211130T144048Z
UID:6637-1636704000-1636736400@www.jingege.wang
SUMMARY:第一次给药
DESCRIPTION:
URL:https://www.jingege.wang/event/%e7%ac%ac%e4%b8%80%e6%ac%a1%e7%bb%99%e8%8d%af/
CATEGORIES:碳化钨
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Shanghai:20210629T080000
DTEND;TZID=Asia/Shanghai:20210629T170000
DTSTAMP:20260406T001321
CREATED:20210628T010450Z
LAST-MODIFIED:20210628T010450Z
UID:5752-1624953600-1624986000@www.jingege.wang
SUMMARY:WB for miR-942 & 徐茜
DESCRIPTION:WB
URL:https://www.jingege.wang/event/wb-for-mir-942-%e5%be%90%e8%8c%9c/
CATEGORIES:YTHDC2
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Shanghai:20210419T110000
DTEND;TZID=Asia/Shanghai:20210419T170000
DTSTAMP:20260406T001321
CREATED:20210418T234426Z
LAST-MODIFIED:20210418T234442Z
UID:5493-1618830000-1618851600@www.jingege.wang
SUMMARY:酶切连接转化
DESCRIPTION:for TNS1 UTR
URL:https://www.jingege.wang/event/%e9%85%b6%e5%88%87%e8%bf%9e%e6%8e%a5%e8%bd%ac%e5%8c%96/
CATEGORIES:YTHDC2
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Shanghai:20210419T080000
DTEND;TZID=Asia/Shanghai:20210419T170000
DTSTAMP:20260406T001321
CREATED:20210418T235106Z
LAST-MODIFIED:20210418T235136Z
UID:5497-1618819200-1618851600@www.jingege.wang
SUMMARY:加压筛选--嘌呤霉素
DESCRIPTION:lentivirus感染H1299细胞 \nluciferase/cadm3-AS1 \n稳定细胞株构建：第二讲
URL:https://www.jingege.wang/event/%e5%8a%a0%e5%8e%8b%e7%ad%9b%e9%80%89-%e5%98%8c%e5%91%a4%e9%9c%89%e7%b4%a0/
CATEGORIES:YTHDC2
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Shanghai:20210416T170000
DTEND;TZID=Asia/Shanghai:20210416T173000
DTSTAMP:20260406T001321
CREATED:20210413T085810Z
LAST-MODIFIED:20210413T085810Z
UID:5482-1618592400-1618594200@www.jingege.wang
SUMMARY:收72hr lentivirus
DESCRIPTION:
URL:https://www.jingege.wang/event/%e6%94%b672hr-lentivirus/
CATEGORIES:YTHDC2
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Shanghai:20210416T090000
DTEND;TZID=Asia/Shanghai:20210416T110000
DTSTAMP:20260406T001321
CREATED:20210413T090119Z
LAST-MODIFIED:20210413T090119Z
UID:5486-1618563600-1618570800@www.jingege.wang
SUMMARY:Dual-luciferase检测
DESCRIPTION:
URL:https://www.jingege.wang/event/dual-luciferase%e6%a3%80%e6%b5%8b/
CATEGORIES:GAPDH
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Shanghai:20210415T170000
DTEND;TZID=Asia/Shanghai:20210415T173000
DTSTAMP:20260406T001321
CREATED:20210413T085738Z
LAST-MODIFIED:20210413T085738Z
UID:5480-1618506000-1618507800@www.jingege.wang
SUMMARY:收48 hr lentivirus
DESCRIPTION:
URL:https://www.jingege.wang/event/%e6%94%b648-hr-lentivirus/
CATEGORIES:YTHDC2
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Shanghai:20210414T100000
DTEND;TZID=Asia/Shanghai:20210414T110000
DTSTAMP:20260406T001321
CREATED:20210413T085947Z
LAST-MODIFIED:20210413T085947Z
UID:5484-1618394400-1618398000@www.jingege.wang
SUMMARY:Dual-luciferase转染
DESCRIPTION:293T细胞
URL:https://www.jingege.wang/event/dual-luciferase%e8%bd%ac%e6%9f%93/
CATEGORIES:GAPDH
END:VEVENT
BEGIN:VEVENT
DTSTART;VALUE=DATE:20210413
DTEND;VALUE=DATE:20210416
DTSTAMP:20260406T001321
CREATED:20210413T090314Z
LAST-MODIFIED:20210413T090314Z
UID:5490-1618272000-1618531199@www.jingege.wang
SUMMARY:文章revise
DESCRIPTION:
URL:https://www.jingege.wang/event/%e6%96%87%e7%ab%a0revise/
CATEGORIES:YTHDC2
END:VEVENT
BEGIN:VEVENT
DTSTART;VALUE=DATE:20210323
DTEND;VALUE=DATE:20210324
DTSTAMP:20260406T001321
CREATED:20210323T003227Z
LAST-MODIFIED:20210323T003227Z
UID:5450-1616457600-1616543999@www.jingege.wang
SUMMARY:TBSTool分析-小程序制作
DESCRIPTION:
URL:https://www.jingege.wang/event/tbstool%e5%88%86%e6%9e%90-%e5%b0%8f%e7%a8%8b%e5%ba%8f%e5%88%b6%e4%bd%9c/
CATEGORIES:GAPDH
END:VEVENT
BEGIN:VEVENT
DTSTART;VALUE=DATE:20210323
DTEND;VALUE=DATE:20210405
DTSTAMP:20260406T001321
CREATED:20210321T164215Z
LAST-MODIFIED:20210321T164215Z
UID:5443-1616457600-1617580799@www.jingege.wang
SUMMARY:构建慢病毒载体
DESCRIPTION:ZNRD1-AS1 \nYTHDC2 \nFOXM1 \n…
URL:https://www.jingege.wang/event/%e6%9e%84%e5%bb%ba%e6%85%a2%e7%97%85%e6%af%92%e8%bd%bd%e4%bd%93/
CATEGORIES:GAPDH,YTHDC2
END:VEVENT
BEGIN:VEVENT
DTSTART;VALUE=DATE:20210323
DTEND;VALUE=DATE:20210326
DTSTAMP:20260406T001321
CREATED:20210321T164038Z
LAST-MODIFIED:20210321T164038Z
UID:5441-1616457600-1616716799@www.jingege.wang
SUMMARY:YTHDC2 review edit
DESCRIPTION:
URL:https://www.jingege.wang/event/ythdc2-review-edit/
CATEGORIES:YTHDC2
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Shanghai:20210117T230000
DTEND;TZID=Asia/Shanghai:20210118T010000
DTSTAMP:20260406T001321
CREATED:20210114T214302Z
LAST-MODIFIED:20210114T214341Z
UID:5199-1610924400-1610931600@www.jingege.wang
SUMMARY:连接TSS和PGL3-转化
DESCRIPTION:Kpn1/HindIII双酶切pGL3/TSS–>连接–>转化
URL:https://www.jingege.wang/event/%e8%bf%9e%e6%8e%a5tss%e5%92%8cpgl3/
CATEGORIES:GAPDH
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Shanghai:20210114T230000
DTEND;TZID=Asia/Shanghai:20210115T010000
DTSTAMP:20260406T001321
CREATED:20210113T080017Z
LAST-MODIFIED:20210113T080017Z
UID:5194-1610665200-1610672400@www.jingege.wang
SUMMARY:RNA stability analysis
DESCRIPTION:
URL:https://www.jingege.wang/event/rna-stability-analysis/
CATEGORIES:YTHDC2
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Shanghai:20210114T180000
DTEND;TZID=Asia/Shanghai:20210114T210000
DTSTAMP:20260406T001321
CREATED:20210113T014036Z
LAST-MODIFIED:20210113T014036Z
UID:5187-1610647200-1610658000@www.jingege.wang
SUMMARY:PCR for GAPDH_TSS
DESCRIPTION:
URL:https://www.jingege.wang/event/pcr-for-gapdh_tss/
CATEGORIES:GAPDH
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Shanghai:20210107T170000
DTEND;TZID=Asia/Shanghai:20210108T010000
DTSTAMP:20260406T001321
CREATED:20210106T094548Z
LAST-MODIFIED:20210106T094548Z
UID:5160-1610038800-1610067600@www.jingege.wang
SUMMARY:WB for Gapdh
DESCRIPTION:
URL:https://www.jingege.wang/event/wb-for-gapdh/
CATEGORIES:GAPDH
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Shanghai:20210106T170000
DTEND;TZID=Asia/Shanghai:20210107T010000
DTSTAMP:20260406T001321
CREATED:20210106T094221Z
LAST-MODIFIED:20210106T094221Z
UID:5156-1609952400-1609981200@www.jingege.wang
SUMMARY:IHC for TTF1/P63/ki67
DESCRIPTION:
URL:https://www.jingege.wang/event/ihc-for-ttf1-p63-ki67/
CATEGORIES:SWCNTs
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Shanghai:20201216T160000
DTEND;TZID=Asia/Shanghai:20201217T010000
DTSTAMP:20260406T001321
CREATED:20201211T075648Z
LAST-MODIFIED:20201211T075648Z
UID:4892-1608134400-1608166800@www.jingege.wang
SUMMARY:MeRIP免疫共沉淀
DESCRIPTION:
URL:https://www.jingege.wang/event/merip%e5%85%8d%e7%96%ab%e5%85%b1%e6%b2%89%e6%b7%80/
CATEGORIES:YTHDC2
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Shanghai:20201213T160000
DTEND;TZID=Asia/Shanghai:20201214T010000
DTSTAMP:20260406T001321
CREATED:20201211T075516Z
LAST-MODIFIED:20201211T075516Z
UID:4890-1607875200-1607907600@www.jingege.wang
SUMMARY:MeRIP样本准备
DESCRIPTION:2B，H1299，A549 分盘–>提total RNA
URL:https://www.jingege.wang/event/merip%e6%a0%b7%e6%9c%ac%e5%87%86%e5%a4%87/
CATEGORIES:YTHDC2
END:VEVENT
END:VCALENDAR