BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//王进的个人网站 - ECPv5.10.0//NONSGML v1.0//EN
CALSCALE:GREGORIAN
METHOD:PUBLISH
X-WR-CALNAME:王进的个人网站
X-ORIGINAL-URL:https://www.jingege.wang
X-WR-CALDESC:Events for 王进的个人网站
BEGIN:VTIMEZONE
TZID:Asia/Shanghai
BEGIN:STANDARD
TZOFFSETFROM:+0800
TZOFFSETTO:+0800
TZNAME:CST
DTSTART:20210101T000000
END:STANDARD
END:VTIMEZONE
BEGIN:VEVENT
DTSTART;TZID=Asia/Shanghai:20220201T080000
DTEND;TZID=Asia/Shanghai:20220201T170000
DTSTAMP:20260418T030626
CREATED:20211130T145132Z
LAST-MODIFIED:20211130T145319Z
UID:6657-1643702400-1643734800@www.jingege.wang
SUMMARY:停止给药8周
DESCRIPTION:各组大鼠分别于染毒结束5天、4周和8周后\,用10 %水合氯醛麻醉后经腹主动脉放血处死。\n6只
URL:https://www.jingege.wang/event/%e5%81%9c%e6%ad%a2%e7%bb%99%e8%8d%af8%e5%91%a8/
CATEGORIES:碳化钨
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Shanghai:20220107T130000
DTEND;TZID=Asia/Shanghai:20220107T170000
DTSTAMP:20260418T030626
CREATED:20211130T145025Z
LAST-MODIFIED:20220105T021959Z
UID:6655-1641560400-1641574800@www.jingege.wang
SUMMARY:停止给药4周
DESCRIPTION:各组大鼠分别于染毒结束5天、4周和8周后\,用10 %水合氯醛麻醉后经腹主动脉放血处死。\n6只
URL:https://www.jingege.wang/event/%e5%81%9c%e6%ad%a2%e7%bb%99%e8%8d%af4%e5%91%a8/
CATEGORIES:碳化钨
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Shanghai:20211212T080000
DTEND;TZID=Asia/Shanghai:20211212T170000
DTSTAMP:20260418T030626
CREATED:20211130T144834Z
LAST-MODIFIED:20211209T101550Z
UID:6653-1639296000-1639328400@www.jingege.wang
SUMMARY:停止给药5天
DESCRIPTION:工作安排： \n1、  水合氯醛麻醉（8%，1.5ml），大鼠称重记录； \n2、  静脉采血：2ml 柠檬酸钠抗凝管用于FACS 血常规分析裂红后加冻存液冻存 \n5ml 于血清管分血清用于血生化（ELISA）-80℃保存 \n3、  腹主动脉放血； \n4、  BALF样本制备： \n1)    打开胸腔，暴露肺；用细线结扎右主支气管； \n2)    剪开颈部皮肤，钝性剥离肌肉，找到气管；（需准备剪刀，镊子，止血钳） \n3)    用细线结扎近口端气管； \n4)    用注射器针头刺穿气管，小心插入预制导管，进气管约1cm，用细线扎住导管以稳定； \n5)    注射器吸20ml PBS缓冲液，将 PBS慢慢注入大鼠肺脏，见左肺膨起，缓慢回抽，反复5次，回收BALF至EP管，冰浴； \n6)    在4 ℃下以1500 r/min离心15 min，取上清于-20 ℃冰箱保存备用，如须保存3个月以上，则需于-70℃冰箱储存； \n7)    上清液用做可溶性成分（白蛋白、总蛋白、IL-6和TNF-α）的检测； \n8)    细胞沉淀加入2 ml细胞冻存液，保存于-80℃冰箱，后复苏用于流式分类计数。 \n  \n  \n5、  器官组织样本 \n1)    大鼠处死后，用无菌眼科剪分离肺脏、心脏、肝脏、脾脏、肾脏、胸腺、睾丸； \n吸取器官表面的血污后，称取器官重量，记录数据； \n2)    剪取约0.2- 0.5 g准确称重后置于EP管中，保存于-20 ℃冰箱中，用于测量器官内的钨浓度。 \n\n大鼠编号： \n对照组：1101-1106 \n实验组：2101-2106
URL:https://www.jingege.wang/event/%e5%81%9c%e6%ad%a2%e7%bb%99%e8%8d%af5%e5%a4%a9/
CATEGORIES:碳化钨
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Shanghai:20211207T080000
DTEND;TZID=Asia/Shanghai:20211207T170000
DTSTAMP:20260418T030626
CREATED:20211130T144356Z
LAST-MODIFIED:20211130T144356Z
UID:6651-1638864000-1638896400@www.jingege.wang
SUMMARY:第八次给药
DESCRIPTION:
URL:https://www.jingege.wang/event/%e7%ac%ac%e5%85%ab%e6%ac%a1%e7%bb%99%e8%8d%af/
CATEGORIES:碳化钨
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Shanghai:20211203T080000
DTEND;TZID=Asia/Shanghai:20211203T170000
DTSTAMP:20260418T030626
CREATED:20211130T144326Z
LAST-MODIFIED:20211130T144326Z
UID:6649-1638518400-1638550800@www.jingege.wang
SUMMARY:第七次给药
DESCRIPTION:
URL:https://www.jingege.wang/event/%e7%ac%ac%e4%b8%83%e6%ac%a1%e7%bb%99%e8%8d%af/
CATEGORIES:碳化钨
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Shanghai:20211130T080000
DTEND;TZID=Asia/Shanghai:20211130T170000
DTSTAMP:20260418T030626
CREATED:20211130T144303Z
LAST-MODIFIED:20211130T144303Z
UID:6647-1638259200-1638291600@www.jingege.wang
SUMMARY:第六次给药
DESCRIPTION:
URL:https://www.jingege.wang/event/%e7%ac%ac%e5%85%ad%e6%ac%a1%e7%bb%99%e8%8d%af/
CATEGORIES:碳化钨
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Shanghai:20211126T080000
DTEND;TZID=Asia/Shanghai:20211126T170000
DTSTAMP:20260418T030626
CREATED:20211130T144241Z
LAST-MODIFIED:20211130T144241Z
UID:6645-1637913600-1637946000@www.jingege.wang
SUMMARY:第五次给药
DESCRIPTION:
URL:https://www.jingege.wang/event/%e7%ac%ac%e4%ba%94%e6%ac%a1%e7%bb%99%e8%8d%af/
CATEGORIES:碳化钨
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Shanghai:20211123T080000
DTEND;TZID=Asia/Shanghai:20211123T170000
DTSTAMP:20260418T030626
CREATED:20211130T144208Z
LAST-MODIFIED:20211130T144208Z
UID:6643-1637654400-1637686800@www.jingege.wang
SUMMARY:第四次给药
DESCRIPTION:
URL:https://www.jingege.wang/event/%e7%ac%ac%e5%9b%9b%e6%ac%a1%e7%bb%99%e8%8d%af/
CATEGORIES:碳化钨
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Shanghai:20211119T080000
DTEND;TZID=Asia/Shanghai:20211119T170000
DTSTAMP:20260418T030626
CREATED:20211130T144139Z
LAST-MODIFIED:20211130T144139Z
UID:6641-1637308800-1637341200@www.jingege.wang
SUMMARY:第一次给药
DESCRIPTION:
URL:https://www.jingege.wang/event/%e7%ac%ac%e4%b8%80%e6%ac%a1%e7%bb%99%e8%8d%af-2/
CATEGORIES:碳化钨
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Shanghai:20211116T080000
DTEND;TZID=Asia/Shanghai:20211116T170000
DTSTAMP:20260418T030626
CREATED:20211130T144117Z
LAST-MODIFIED:20211130T144117Z
UID:6639-1637049600-1637082000@www.jingege.wang
SUMMARY:第二次给药
DESCRIPTION:
URL:https://www.jingege.wang/event/%e7%ac%ac%e4%ba%8c%e6%ac%a1%e7%bb%99%e8%8d%af/
CATEGORIES:碳化钨
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Asia/Shanghai:20211112T080000
DTEND;TZID=Asia/Shanghai:20211112T170000
DTSTAMP:20260418T030626
CREATED:20211130T144048Z
LAST-MODIFIED:20211130T144048Z
UID:6637-1636704000-1636736400@www.jingege.wang
SUMMARY:第一次给药
DESCRIPTION:
URL:https://www.jingege.wang/event/%e7%ac%ac%e4%b8%80%e6%ac%a1%e7%bb%99%e8%8d%af/
CATEGORIES:碳化钨
END:VEVENT
END:VCALENDAR