Knowing the frequency of cells in your flow assay is important to enable acquisition of sufficient cells.
These handy reference tables for human, mouse, and rat tissues, commonly used in flow cytometry, will help determine starting cell numbers for staining and cell sorting, allowing you to optimize your experiments.
了解流动分析中细胞的比例对于获取足够的细胞非常重要。
这些方便的人类，小鼠和大鼠组织参考表，通常用于流式细胞仪，将有助于确定染色和细胞分选的起始细胞数量，使您能够优化实验。
转载自Bio-Rad官网：flow-cytometry-cell-frequency.pdf
Table 1. Human cell frequency in peripheral blood.
|
Leukocytes |
Lymphoid Cells |
Myeloid Cells |
|||
|
Cell Type |
Percent |
Cell Type |
Percent |
Cell Type |
Percent |
|
T cells |
10–25 |
CD4+ |
10–20 |
Neutrophils |
40–65 |
|
B cells |
3–10 |
CD8+ |
3–10 |
Eosinophils |
0.1–2 |
|
Granulocytes |
45–65 |
Tregs |
0.2–1.4 |
Basophils |
0.1–2 |
|
Monocytes |
3–10 |
γδ T cells |
1–5 |
Dendritic cells |
0.5–1 |
|
NK cells |
2–5 |
Naïve B cells |
0.5–5 |
Monocyte DCs |
0.2–0.4 |
|
Dendritic cells (DC) |
0.5–1 |
Memory B cells |
0.4–2 |
Plasmacytoid DCs |
0.1–0.2 |
|
Stem cells |
0.01–0.05 |
NKT cells |
0.01–0.5 |
Innate lymphoid cells |
0.01–0.1 |
Table 2. Mouse cell frequency.
|
Spleen |
Thymus |
Peripheral Blood |
Lymph Node |
||||
|
Cell Type |
Percent |
Cell Type |
Percent |
Cell Type |
Percent |
Cell Type |
Percent |
|
T cells |
21–35 |
CD4+ |
4–6 |
T cells |
17–20 |
T cells |
65–78 |
|
CD4+ |
13–20 |
CD8+ |
1–2 |
CD4+ |
8–12 |
CD4+ |
35–64 |
|
CD8+ |
7–15 |
CD4+/8+ |
85–95 |
CD8+ |
7–10 |
CD8+ |
19–30 |
|
γδ T cells |
0.5–1 |
B cells |
35–58 |
B cells |
9–15 |
||
|
B cells |
44–58 |
Monocytes |
2–3 |
Tregs |
2–3 |
||
|
Monocytes |
3.5–5 |
Neutrophils |
4–6 |
Dendritic cells |
0.5–1 |
||
|
Granulocytes |
1–2 |
Eosinophils |
1–2 |
||||
|
NK cells |
1–5 |
NK cells |
4–7 |
||||
|
iNKT |
1–2 |
iNKT cells |
0.2–0.5 |
||||
|
Myeloid DC |
0.5–2 |
||||||
|
Lymphoid DC |
0.5–1 |
||||||
|
Plasmacytoid DC |
0.1–0.3 |
||||||
Table 3. Rat cell frequency.
|
Spleen |
Thymus |
Peripheral Blood |
|||
|
Cell Type |
Percent |
Cell Type |
Percent |
Cell Type |
Percent |
|
T cells |
31.5–33.5 |
CD4+ |
5.7–9.4 |
T cells |
51.5–66.5 |
|
CD4+ |
21.5–26.5 |
CD8+ |
2.3–6.9 |
CD4+ |
32–40 |
|
CD8+ |
14–18 |
CD4+/8+ |
83–87.6 |
CD8+ |
18–26 |
|
B cells |
51–59 |
CD4-/8- |
1.4–2.2 |
B cells |
22.7–40.7 |
|
Monocytes |
1–6 |
||||
|
Neutrophils |
14–20 |
||||
|
Eosinophils |
1–4 |
||||
|
Basophils |
Rare |
||||
|
γδ T cells |
1–2 |
||||
|
NK cells |
6–10 |
||||
同周龄的雌鼠和雄鼠外周血细胞比例会有差异吗？