修改MRI_Wound_Healing_Tool.ijm细胞划痕插件，实现批处理，相关参数设定参考插件说明http://dev.mri.cnrs.fr/wiki/imagej-macros/Wound_Healing_Tool

将下面代码保存为txt或ijm，imageJ里面Macro–>Plugins–>Macros–>Run–>保存的脚本，然后选择图片所在目录，软件会自动分析。

可能有些图片会有多个结果，原因是细胞过于稀疏或者有空白，也或者划痕区域有杂点等，这种情况下可用插件确认问题，手动进行分析。

软件毕竟不是人脑，程序化操作不可尽信，有疑惑可以double check。

var helpURL = "http://dev.mri.cnrs.fr/wiki/imagej-macros/Wound_Healing_Tool"
var varianceFilterRadius = 20;
var threshold = 50;
var radiusOpen = 4;
var minSize = 10000;
var methods = newArray("variance", "find edges");
var method = "variance";
var measureInPixelUnits = false;

function thresholdVariance() {
    run("Variance...", "radius=" + varianceFilterRadius + " stack");
    run("8-bit");
    setThreshold(0,threshold);
}

function thresholdFindEdges() {
    run("Find Edges", "stack");
    run("Invert", "stack");
    setAutoThreshold("Percentile dark");
}

dir=getDirectory("Select the source directory");
list=getFileList(dir);
Array.sort(list);
###修改划痕图片对应格式，此处为jpg，其它对应修改
for(i=0;i<list.length;i++){
	filename=dir+list[i];
	if(endsWith(filename,"jpg")){
		open(filename);
	run("Select None");
	getPixelSize(unit, pixelWidth, pixelHeight);
	run("Duplicate...", "duplicate");
    setForegroundColor(0, 0, 0);
    setBackgroundColor(255, 255, 255);
    roiManager("reset")
    roiManager("Associate", "true");
    if (method=="variance") 
        thresholdVariance();
    else 
        thresholdFindEdges();
    run("Convert to Mask", " black");
    resetThreshold();
    run("Invert", "stack");
    for (j=0; j<radiusOpen; j++) {
        run("Dilate", "stack");
    }
    for (j=0; j<radiusOpen; j++) {
        run("Erode", "stack");
    }
    run("Select All");
    run("Enlarge...", "enlarge=-" + radiusOpen + " pixel");
    run("Invert", "stack");
    run("Analyze Particles...", "size="+minSize+"-Infinity circularity=0.00-1.00 show=Nothing add stack");
    close();
    if (measureInPixelUnits)
    		run("Set Scale...", "distance=0 known=0 pixel=1 unit=pixel");
    roiManager("Measure"); 
    roiManager("Show None");
    roiManager("Show All");
    close();
}
}

手把手教你用Image J测量细胞划痕面积