1、Luciferase 概念
荧光素酶报告基因是指以荧光素(luciferin)为底物来检测萤火虫荧光素酶(fireflyluciferase)活性的一种报告系统。
![](https://www.jingege.wang/wp-content/uploads/2020/09/2020092202452698272893346.jpg)
2、Luciferase 作用
荧光素酶报告基因系统可高效的检测miRNA是否与目的基因启动子区及3’UTR区相互作用。
![](https://www.jingege.wang/wp-content/uploads/2020/09/2020092202452729242306579.png)
荧光信号强度=荧光素酶基因表达=启动子活性
![](https://www.jingege.wang/wp-content/uploads/2020/09/2020092202452772626882968.png)
![](https://www.jingege.wang/wp-content/uploads/2020/09/2020092202452858366083670.jpg)
1、双荧光素酶报告系统
![](https://www.jingege.wang/wp-content/uploads/2020/09/2020092202453058492897350.png)
![](https://www.jingege.wang/wp-content/uploads/2020/09/2020092202453032663144299.png)
荧光信号有无检测转录因子及miRNA与目的基因启动子区域调控作用。
![](https://www.jingege.wang/wp-content/uploads/2020/09/2020092202453069462671763.png)
荧光信号强弱检测 miRNA 与目的基因,UTR区调控作用。
![](https://www.jingege.wang/wp-content/uploads/2020/09/2020092202453091091313129.png)
1、Luciferase 设计原理
![](https://www.jingege.wang/wp-content/uploads/2020/09/2020092202453110747116183.jpg)
2、Luciferase 注意事项
靶向关系预测,并PCR检测
![](https://www.jingege.wang/wp-content/uploads/2020/09/2020092202453145733492687.png)
![](https://www.jingege.wang/wp-content/uploads/2020/09/2020092202453165911953556.png)
![](https://www.jingege.wang/wp-content/uploads/2020/09/2020092202453175751381669.png)
![](https://www.jingege.wang/wp-content/uploads/2020/09/2020092202453212140473070.png)